SMAD3 antibody (C-Term)

Catalog No. ABIN129676

Product Details anti-SMAD3 Antibody

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Xenopus laevis, Xenopus tropicalis, Zebrafish (Brachydanio rerio), Rat (Rattus), Mouse (Murine), Pig (Porcine), Sheep (Ovine), Chicken

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the domain of human SMAD3 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.  This reagent reacts equally to phosphorylated and non-phosphorylated Smad3 by ELISA and western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.17 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-SMAD3 antibody (ABIN129676)

Katsuno, Meyer, Zhang, Shokat, Akhurst, Miyazono, Derynck: "Chronic TGF-β exposure drives stabilized EMT, tumor stemness, and cancer drug resistance with vulnerability to bitopic mTOR inhibition." in: Science signaling, Vol. 12, Issue 570, (2019) (PubMed).

Sysa, Potter, Liu, Mezey: "Transforming growth factor-beta1 up-regulation of human alpha(1)(I) collagen is mediated by Sp1 and Smad2 transacting factors." in: DNA and cell biology, Vol. 28, Issue 9, pp. 425-34, (2009) (PubMed).

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3 (SMAD3 Products)

Synonyms: HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-ß superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-ß-mediated response.
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody

Gene ID: 4088, 5174513

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy