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RFP antibody (HRP)

RFP Reactivity: Discosoma WB, ELISA, IF Host: Rabbit Polyclonal HRP
Catalog No. ABIN129725
  • Target See all RFP Antibodies
    RFP (Red Fluorescent Protein (RFP))
    Reactivity
    • 51
    • 1
    Discosoma
    Host
    • 22
    • 14
    • 9
    • 5
    • 3
    Rabbit
    Clonality
    • 31
    • 22
    Polyclonal
    Conjugate
    • 20
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is conjugated to HRP
    Application
    • 27
    • 21
    • 14
    • 12
    • 10
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Immunogen
    The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
    Immunogentype:Recombinant
    Isotype
    IgG
    Featured
    Discover our best selling RFP Primary Antibody
  • Application Notes
    Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Gentamicin sulfate
    Handling Advice
    Do NOT add Sodium Azide!
    Storage
    4 °C
  • Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." in: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

  • Target
    RFP (Red Fluorescent Protein (RFP))
    Alternative Name
    RFP (RFP Products)
    Background
    Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
    Synonyms: HRP, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
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