CoREST antibody (AA 109-293)

Catalog No. ABIN1304609

The Mouse Monoclonal anti-CoREST antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect CoREST in samples from Human.

Quick Overview for CoREST antibody (AA 109-293) (ABIN1304609)

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CoREST antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: K72-8

Product Details anti-CoREST Antibody

Binding Specificity: AA 109-293

Specificity: No off-targets reported

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-Co-Rest/RCOR1 mouse monoclonal primary antibody is produced in-house from hybridoma clone K72/8. It detects human, mouse, and rat Co-Rest/RCOR1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our Co-Rest/RCOR1 mouse monoclonal primary antibody from hybridoma clone K72/8. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 109-293 of human Co-Rest (accession number Q9UKL0), includes ELM2 and SANT domains, produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for CoREST

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Alternative Name: RCOR1

Background:

Synonyms: REST corepressor 1 (Protein CoREST)

Target Description: REST Corepressor 1, CoREST or RCOR is encoded by the gene RCOR1 and is a member of the CoREST family of proteins. CoREST is a corepressor to the RE1 silencing transcription factor/neural restrictive silencing (REST) transcription factor and is part of a multiprotein complex (BHC complex) that represses transcription of neuron-specific genes in non-neuronal cells. This complex determines neural cell differentiation. CoREST is ubiquitously expressed in the nucleus of cells. Diseases associated with this gene include Huntington Disease and Loeys-Dietz Syndrome 1.

Gene Name Alternatives: RCOR1 KIAA0071 RCOR

Molecular Weight: 66-80 kDa (depending on gel system used)

NCBI Accession: NP_055971

Pathways: Regulation of Hormone Metabolic Process, Chromatin Binding