GRIK1 antibody (AA 824-1203)

Catalog No. ABIN1304821

This Mouse Monoclonal antibody specifically detects GRIK1 in WB, IHC, ICC and IP. It exhibits reactivity toward Rat.

Quick Overview for GRIK1 antibody (AA 824-1203) (ABIN1304821)

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: N75-3

Product Details anti-GRIK1 Antibody

Binding Specificity: AA 824-1203

Specificity: Recognizes mGluR1 and mGluR5

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-mGluR1/5 (Group I) glutamate receptor mouse monoclonal primary antibody is produced in-house from hybridoma clone N75/3. It detects human, mouse, and rat mGluR1/5 (Group I) glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our mGluR1/5 (Group I) glutamate receptor mouse monoclonal primary antibody from hybridoma clone N75/3. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession number P31424) produced recombinantly in E. Coli

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Alternative Name: 5 glutamate receptor

Background:

Synonyms: Metabotropic glutamate receptor 5 (mGluR5)

Target Description: The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004, Francesconi et al., 2004, Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Gene Name Alternatives: Grm5 Gprc1e Mglur5

Molecular Weight: 130 kDa (both mGluR1 and mGluR5)

UniProt: P31424

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity