GRIK1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886370

The Rabbit Polyclonal anti-GRIK1 antibody is suitable to detect GRIK1 in samples from Rat. It has been validated for WB, IHC, IF, IC and LCI.

Quick Overview for GRIK1 antibody (Extracellular, N-Term) (ABIN7886370)

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-GRIK1 Antibody

Binding Specificity: Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Kainate Receptor GluK1

Predicted Reactivity: Human,dog,monkey - 15,16 amino acid residues identical

Characteristics: Anti-GRIK1 (GluK1) (extracellular) Antibody (ABIN7043237, ABIN7044334 and ABIN7044335) is directed against the extracellular N-terminus domain of the rat kainate receptor GluK1. The antibody can be used in western blot and immunofluorescence applications.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KASGEVSKHLYKVWKK, corresponding to amino acid residues 402-417 of rat kainate receptor GluK1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235653)

Blocking Peptide: (ABIN7235653)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Alternative Name: GRIK1

Background:

Synonyms: Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5

Description: L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing, as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2GluR5 is highly expressed in dorsal root ganglion (DRG) neurons of the peripheral nervous system (PNS) as well as in several structures of the CNS including the amygdala, the hipoccampus, and Purkinje cells of the cerebellum.GluR5 has been implicated in the modulation of synaptic transmission and synaptic plasticity in both the CNS and PNS, but the exact physiological function of GluR5 is not well understood. Nevertheless, an involvement in persistent pain and some types of learning has been proposed.

Gene ID: 29559

NCBI Accession: NM_000830

UniProt: P22756

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity