Bcl-2 antibody (AA 61-76)

Catalog No. ABIN135029

The Mouse Monoclonal anti-Bcl-2 antibody has been validated for WB. It is suitable to detect Bcl-2 in samples from Mouse and Rat.

Quick Overview for Bcl-2 antibody (AA 61-76) (ABIN135029)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 10C4

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 61-76

Specificity: Mouse/Rat Bcl-2

Characteristics: Mouse Anti-Bcl-2-UNLB

Purification: Purified

Immunogen: Murine Bcl-2 peptide (a.a. 61-76)

Isotype: IgG1

Application Details

Application Notes:

• Applications: WB - Quality tested , IP - Reported in literature , FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature
• Working Dilutions: Immunoblotting Purified (UNLB) antibody ≤ 2 g/mL

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background: Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, -irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection.

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling