CD4 antibody (SPRD)

Catalog No. ABIN135104

The Rat Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Mouse.

Quick Overview for CD4 antibody (SPRD) (ABIN135104)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to SPRD

Application: Flow Cytometry (FACS)

Clone: GK1-5

Product Details anti-CD4 Antibody (SPRD)

Brand: Spectral Red ™

Specificity: Mouse CD4, Mr 55 kDa

Characteristics: Rat Anti-Mouse CD4-SPRD

Immunogen: Mouse CTL clone V4

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Sep - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE, APC, PE/TXRD, SPRD, CY5, PE/CY5.5, PE/CY7, 0.2 g/106 cells APC/CY5.5, APC/CY7, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events