CD11b antibody (SPRD)

Catalog No. ABIN135140

The Rat Monoclonal anti-CD11b antibody has been validated for FACS. It is suitable to detect CD11b in samples from Mouse.

Quick Overview for CD11b antibody (SPRD) (ABIN135140)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to SPRD

Application: Flow Cytometry (FACS)

Clone: M1-70

Product Details anti-CD11b Antibody (SPRD)

Brand: Spectral Red ™

Specificity: Mouse/Human/Rhesus/Baboon/Rabbit CD11b, Mr 170 kDa

Characteristics: Rat Anti-Mouse CD11b-SPRD

Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, CY5, PE/CY7, APC/CY7, PACBLU, AF647 and AF700 0.3 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro and in vivo blocking

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation