CD25 antibody

Catalog No. ABIN135179

The Rat Monoclonal anti-CD25 antibody is suitable to detect CD25 in samples from Mouse. It has been validated for FACS, IP, IHC (fro), BR and Neut.

Quick Overview for CD25 antibody (ABIN135179)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Neutralization (Neut)

Clone: 3C7

Product Details anti-CD25 Antibody

Purpose: Rat Anti-Mouse CD25-LE/AF

Specificity: Mouse CD25, Mr 55 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD25 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, blocking, and neutralization assays.

Endotoxin Level: Low Endotoxin

Immunogen: IL-2 dependent BALB/c helper T cell clone HT2

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.

Gene ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation