CTLA4 antibody

Catalog No. ABIN135319

This Hamster Monoclonal antibody specifically detects CTLA4 in FACS, ELISA, IP, BR and CoSt. It exhibits reactivity toward Mouse.

Quick Overview for CTLA4 antibody (ABIN135319)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR), Co-Stimulation (CoSt)

Clone: 9H10

Product Details anti-CTLA4 Antibody

Purpose: Hamster Anti-Mouse CD152-LE/AF

Specificity: Mouse CD152, Mr 33-37 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD152 antibody for use in flow cytometry, ELISA, blocking, and costimulation assays.

Endotoxin Level: Low Endotoxin

Immunogen: Heat-killed Staphylococcus A bacteria coated with mouse CTLA-4/human IgG1 fusion protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152

Background: CD152, also known as CTLA-4, is homologous to the costimulatory molecule CD28. Like CD28, the CD152 molecule binds the family of B7 counter-receptors (CD80/B7-1 and CD86/B7-2) on antigen-presenting cells. CTLA-4 is not expressed on resting T lymphocytes but is induced 2-3 days following T-cell stimulation via the T cell receptor (TCR) complex. Whereas CD28 provides a positive costimulatory signal, CD152/CTLA-4 is a negative regulator of T-cell activation, possibly by inhibiting tyrosine kinase signalling through the TCR through its association with tyrosine phosphatases such as SHP-2.

Gene ID: 12477

UniProt: P09793

Pathways: Cancer Immune Checkpoints