IL2 Receptor beta antibody

Catalog No. ABIN135408

Product Details anti-IL2 Receptor beta Antibody

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2 Receptor beta antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Mouse CD122, 90-100 kDa

Cross-Reactivity (Details): Cross-reactivity with Stat1 from other sources has not been determined.

Predicted Reactivity: A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted.

Characteristics: Rat Anti-Mouse CD122-UNLB

Purification: Purified

Immunogen: Rat myeloma YB2/0 transfected with truncated IL-2Rbeta cDNA (YB2/0-mbetat-28)

Clone: 5H4

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: CD122 (IL2RB Products)

Background: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the chain which binds IL-2 with low affinity, (ii) the chain that binds IL-2 with high affinity, and (ii) the common chain (C) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a kDa of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on 30 % of CD8+ cells and all NK cells, but < 1 % of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.

Pathways: JAK-STAT Signaling, Growth Factor Binding