H2-D1 antibody (Biotin)

Catalog No. ABIN135414

The Biotin-conjugated Mouse Monoclonal anti-H2-D1 antibody (Clone 28-14-8) (ABIN135414) specifically detects H2-D1 in FACS. The antibody is reactive with Mouse samples.

Quick Overview for H2-D1 antibody (Biotin) (ABIN135414)

Target: H2-D1 (Histocompatibility 2, D Region Locus 1 (H2-D1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2-D1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 28-14-8

Product Details anti-H2-D1 Antibody (Biotin)

Specificity: Mouse H-2Db

Characteristics: Mouse Anti-Mouse H-2Db-BIOT

Immunogen: C3H.SW mouse splenocytes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.5 g/106 cells APC conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Complement-mediated cytotoxicity

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for H2-D1

Target: H2-D1 (Histocompatibility 2, D Region Locus 1 (H2-D1))

Alternative Name: H-2Db

Background: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic chains noncovalently associated with the invariant 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process