LAMP1 antibody

Catalog No. ABIN135442

This Rat Monoclonal antibody specifically detects LAMP1 in WB, FACS, IHC and IP. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for LAMP1 antibody (ABIN135442)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 1D4B

Product Details anti-LAMP1 Antibody

Specificity: Mouse CD107a, Mr 110-140 kDa

Characteristics: Rat Anti-Mouse CD107a-UNLB

Purification: Purified

Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line

Isotype: IgG2a

Application Details

Application Notes: Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Comment:

Immunoelectron microscopy, Immunoaffinity chromatography

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Buffer: Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

References for anti-LAMP1 antibody (ABIN135442)

Song, Lee, Schindler: "Deletion of the murine scavenger receptor CD68." in: Journal of lipid research, Vol. 52, Issue 8, pp. 1542-50, (2011) (PubMed).

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: CD107a

Background: CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases.

Pathways: Autophagy