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CD4 antibody (FITC)

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Pig. There are 2+ publications available.
Catalog No. ABIN135473

Quick Overview for CD4 antibody (FITC) (ABIN135473)

Target

See all CD4 Antibodies
CD4

Reactivity

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Pig

Host

  • 426
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  • 126
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Mouse

Clonality

  • 571
  • 154
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Monoclonal

Conjugate

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  • 12
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This CD4 antibody is conjugated to FITC

Application

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Flow Cytometry (FACS)

Clone

74-12-4
  • Specificity

    Porcine CD4, Mr 55 kDa

    Characteristics

    Mouse Anti-Porcine CD4-FITC

    Immunogen

    Fresh dd miniature swine thymocytes

    Isotype

    IgG2b
  • Application Notes

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Block - Reported in literature , CMCD - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Comment

    Cytotoxicity Assays, Blocking proliferative responses

    Sample Volume

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Guo, Fu, Wang, Lu, Wei, Tang, Fan, Liu, Zhang, Zhang, Huang, Liu, Li, Wu, Liu: "A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain." in: PLoS ONE, Vol. 7, Issue 7, pp. e41463, (2012) (PubMed).

    Hosoya, Villena, Shimazu, Tohno, Fujie, Chiba, Shimosato, Aso, Suda, Kawai, Saito, Alvarez, Ikegami, Itoh, Kitazawa: "Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells." in: Veterinary research, Vol. 42, pp. 111, (2011) (PubMed).

  • Target

    CD4

    Alternative Name

    CD4

    Background

    Porcine CD4 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It has a pattern of expression similar to human CD4 and is not expressed on porcine CD8+ T cells. CD4 is an accessory molecule in the recognition of foreign antigens in association with MHC Class II molecules by T lymphocytes.

    Pathways

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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