CD4 antibody (FITC)

Catalog No. ABIN135473

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Pig. There are 2+ publications available.

Quick Overview for CD4 antibody (FITC) (ABIN135473)

Target: CD4

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 74-12-4

Product Details anti-CD4 Antibody (FITC)

Specificity: Porcine CD4, Mr 55 kDa

Characteristics: Mouse Anti-Porcine CD4-FITC

Immunogen: Fresh dd miniature swine thymocytes

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Block - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Cytotoxicity Assays, Blocking proliferative responses

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD4 antibody (FITC) (ABIN135473)

Guo, Fu, Wang, Lu, Wei, Tang, Fan, Liu, Zhang, Zhang, Huang, Liu, Li, Wu, Liu: "A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain." in: PLoS ONE, Vol. 7, Issue 7, pp. e41463, (2012) (PubMed).

Hosoya, Villena, Shimazu, Tohno, Fujie, Chiba, Shimosato, Aso, Suda, Kawai, Saito, Alvarez, Ikegami, Itoh, Kitazawa: "Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells." in: Veterinary research, Vol. 42, pp. 111, (2011) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Porcine CD4 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It has a pattern of expression similar to human CD4 and is not expressed on porcine CD8+ T cells. CD4 is an accessory molecule in the recognition of foreign antigens in association with MHC Class II molecules by T lymphocytes.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events