CD4 antibody

Catalog No. ABIN135500

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Cat. There are 2+ publications available.

Quick Overview for CD4 antibody (ABIN135500)

Target: CD4

Reactivity: Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 3-4F4

Product Details anti-CD4 Antibody

Specificity: Feline/Lion CD4, Mr 65 kDa

Characteristics: Mouse Anti-Feline CD4-UNLB

Purification: Purified

Immunogen: Feline T cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD4 antibody (ABIN135500)

Webb, Quimby, Dow: "In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells." in: Journal of feline medicine and surgery, Vol. 14, Issue 2, pp. 165-8, (2012) (PubMed).

Katrin Helfer-Hungerbuehler, Cattori, Bachler, Hartnack, Riond, Ossent, Lutz, Hofmann-Lehmann: "Quantification and molecular characterization of the feline leukemia virus A receptor." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, (2011) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of peripheral T lymphocytes. It is present on approximately 55 % of thymocytes, 15 % of splenocytes, 40 % of lymph node cells, and 25 % of peripheral blood lymphocytes. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events