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CD4 antibody

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Cat. There are 2+ publications available.
Catalog No. ABIN135500

Quick Overview for CD4 antibody (ABIN135500)

Target

See all CD4 Antibodies
CD4

Reactivity

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Cat

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD4 antibody is un-conjugated

Application

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Flow Cytometry (FACS)

Clone

3-4F4
  • Specificity

    Feline/Lion CD4, Mr 65 kDa

    Characteristics

    Mouse Anti-Feline CD4-UNLB

    Purification

    Purified

    Immunogen

    Feline T cells

    Isotype

    IgG1
  • Application Notes

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Block - Reported in literature
    • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Sample Volume

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

    Preservative

    Without preservative

    Handling Advice

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Webb, Quimby, Dow: "In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells." in: Journal of feline medicine and surgery, Vol. 14, Issue 2, pp. 165-8, (2012) (PubMed).

    Katrin Helfer-Hungerbuehler, Cattori, Bachler, Hartnack, Riond, Ossent, Lutz, Hofmann-Lehmann: "Quantification and molecular characterization of the feline leukemia virus A receptor." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, (2011) (PubMed).

  • Target

    CD4

    Alternative Name

    CD4

    Background

    Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of peripheral T lymphocytes. It is present on approximately 55 % of thymocytes, 15 % of splenocytes, 40 % of lymph node cells, and 25 % of peripheral blood lymphocytes. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

    Pathways

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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