CD4 antibody (PE)

Catalog No. ABIN135503

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Cat.

Quick Overview for CD4 antibody (PE) (ABIN135503)

Target: CD4

Reactivity: Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 3-4F4

Product Details anti-CD4 Antibody (PE)

Specificity: Feline/Lion CD4, Mr 65 kDa

Characteristics: Mouse Anti-Feline CD4-PE

Immunogen: Feline T cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of peripheral T lymphocytes. It is present on approximately 55 % of thymocytes, 15 % of splenocytes, 40 % of lymph node cells, and 25 % of peripheral blood lymphocytes. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events