CD4 antibody (FITC)

Catalog No. ABIN135509

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Chicken. There are 6+ publications available.

Quick Overview for CD4 antibody (FITC) (ABIN135509)

Target: CD4

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: CT-4

Product Details anti-CD4 Antibody (FITC)

Specificity: Chicken/Turkey/Quail/Pigeon CD4, Mr 64 kDa

Characteristics: Mouse Anti-Chicken CD4-FITC

Immunogen: Chicken thymocytes and Ig-negative blood leukocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD4 antibody (FITC) (ABIN135509)

Han, Renu, Patil, Schrock, Feliciano-Ruiz, Selvaraj, Renukaradhya: "Immune Response to Salmonella Enteritidis Infection in Broilers Immunized Orally With Chitosan-Based Salmonella Subunit Nanoparticle Vaccine." in: Frontiers in immunology, Vol. 11, pp. 935, (2020) (PubMed).

Peng, Yu, Liang, Chi, Li, Jiang, Fang, Cui, Lai, Zhou, Zhou: "The mitochondrial and death receptor pathways involved in the thymocytes apoptosis induced by aflatoxin B1." in: Oncotarget, Vol. 7, Issue 11, pp. 12222-34, (2017) (PubMed).

Peng, Zhang, Bai, Ding, Zeng, Yang, Fang, Chen: "Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn." in: International journal of environmental research and public health, Vol. 11, Issue 8, pp. 8567-80, (2015) (PubMed).

Chen, Peng, Fang, Cui, Zuo, Deng, Chen, Geng, Lai, Tang, Yang: "Effects of dietary selenium on histopathological changes and T cells of spleen in broilers exposed to aflatoxin B1." in: International journal of environmental research and public health, Vol. 11, Issue 2, pp. 1904-13, (2015) (PubMed).

Matulova, Stepanova, Sisak, Havlickova, Faldynova, Kyrova, Volf, Rychlik: "Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with salmonella enteritidis." in: PLoS ONE, Vol. 7, Issue 2, pp. e32346, (2012) (PubMed).

Cheng, Zhu, Sun: "The immune enhancement of sodium lauryl sulfoacetate in chickens." in: Veterinary medicine international, Vol. 2010, pp. 485060, (2010) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a type I transmembrane glycoprotein expressed on approximately 70 % of thymocytes, 10 % of spleen cells and 45 % of peripheral blood lymphocytes, but < 1 % of bursal cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events