CD4 antibody (PE)

Catalog No. ABIN135511

This Mouse Monoclonal antibody specifically detects CD4 in FACS. It exhibits reactivity toward Chicken and has been mentioned in 4+ publications.

Quick Overview for CD4 antibody (PE) (ABIN135511)

Target: CD4

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: CT-4

Product Details anti-CD4 Antibody (PE)

Specificity: Chicken/Turkey/Quail/Pigeon CD4, Mr 64 kDa

Characteristics: Mouse Anti-Chicken CD4-PE

Immunogen: Chicken thymocytes and Ig-negative blood leukocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD4 antibody (PE) (ABIN135511)

He, Han, Ding, Zhang, Chang, Zhang, Zhao, Yang, Song: "Linc-GALMD1 Regulates Viral Gene Expression in the Chicken." in: Frontiers in genetics, Vol. 10, pp. 1122, (2019) (PubMed).

Carballeda, Zoth, Gómez, Gravisaco, Berinstein: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, (2011) (PubMed).

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, (2010) (PubMed).

Parvizi, Andrzejewski, Read, Behboudi, Sharif: "Expression profiling of genes associated with regulatory functions of T-cell subsets in Marek's disease virus-infected chickens." in: Avian pathology : journal of the W.V.P.A, Vol. 39, Issue 5, pp. 367-73, (2010) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a type I transmembrane glycoprotein expressed on approximately 70 % of thymocytes, 10 % of spleen cells and 45 % of peripheral blood lymphocytes, but < 1 % of bursal cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events