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CD4 antibody (PE)

This Mouse Monoclonal antibody specifically detects CD4 in FACS. It exhibits reactivity toward Chicken and has been mentioned in 4+ publications.
Catalog No. ABIN135511

Quick Overview for CD4 antibody (PE) (ABIN135511)

Target

See all CD4 Antibodies
CD4

Reactivity

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Chicken

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD4 antibody is conjugated to PE

Application

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Flow Cytometry (FACS)

Clone

CT-4
  • Specificity

    Chicken/Turkey/Quail/Pigeon CD4, Mr 64 kDa

    Characteristics

    Mouse Anti-Chicken CD4-PE

    Immunogen

    Chicken thymocytes and Ig-negative blood leukocytes

    Isotype

    IgG1
  • Application Notes

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Sample Volume

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • He, Han, Ding, Zhang, Chang, Zhang, Zhao, Yang, Song: "Linc-GALMD1 Regulates Viral Gene Expression in the Chicken." in: Frontiers in genetics, Vol. 10, pp. 1122, (2019) (PubMed).

    Carballeda, Zoth, Gómez, Gravisaco, Berinstein: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, (2011) (PubMed).

    Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, (2010) (PubMed).

    Parvizi, Andrzejewski, Read, Behboudi, Sharif: "Expression profiling of genes associated with regulatory functions of T-cell subsets in Marek's disease virus-infected chickens." in: Avian pathology : journal of the W.V.P.A, Vol. 39, Issue 5, pp. 367-73, (2010) (PubMed).

  • Target

    CD4

    Alternative Name

    CD4

    Background

    CD4 is a type I transmembrane glycoprotein expressed on approximately 70 % of thymocytes, 10 % of spleen cells and 45 % of peripheral blood lymphocytes, but < 1 % of bursal cells.

    Pathways

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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