CD4 antibody

Catalog No. ABIN135725

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Human.

Quick Overview for CD4 antibody (ABIN135725)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: RFT4

Product Details anti-CD4 Antibody

Specificity: Human CD4, Mr 55 kDa

Characteristics: Mouse Anti-Human CD4-UNLB

Purification: Purified

Immunogen: Thymocyte and E-rosetted lymphocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Sep - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, PE/TXRD, APC, SPRD, CY5, PE/CY5.5, 10 L/106 cells PE/CY7, APC/CY7, AF488, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: D4 is a 59 kDa type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the helper/inducer subpopulation of mature T lymphocytes, and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessary molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events