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CD4 antibody

This anti-CD4 antibody is a Mouse Monoclonal antibody detecting CD4 in FACS. Suitable for Human.
Catalog No. ABIN135729

Quick Overview for CD4 antibody (ABIN135729)

Target

See all CD4 Antibodies
CD4

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD4 antibody is un-conjugated

Application

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Flow Cytometry (FACS)

Clone

RFT4
  • Specificity

    Human CD4, Mr 55 kDa

    Characteristics

    Mouse Anti-Human CD4-LE/AF

    Endotoxin Level

    Low endotoxin

    Immunogen

    Thymocyte and E-rosetted lymphocytes

    Isotype

    IgG1
  • Application Notes

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Sep - Reported in literature
    • Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, PE/TXRD, APC, SPRD, CY5, PE/CY5.5, 10 L/106 cells PE/CY7, APC/CY7, AF488, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Sample Volume

    0,2 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg of purified immunoglobulin in 1.0 mL of PBS

    Preservative

    Azide free

    Handling Advice

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    -20 °C

    Storage Comment

    Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
  • Target

    CD4

    Alternative Name

    CD4

    Background

    D4 is a 59 kDa type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the helper/inducer subpopulation of mature T lymphocytes, and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessary molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

    Pathways

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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