CD14 antibody

Catalog No. ABIN135743

The Mouse Monoclonal anti-CD14 antibody has been validated for FACS. It is suitable to detect CD14 in samples from Human.

Quick Overview for CD14 antibody (ABIN135743)

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: UCHM-1

Product Details anti-CD14 Antibody

Specificity: Human/Cynomolgus CD14, Mr 55 kDa

Characteristics: Mouse Anti-Human CD14-UNLB

Purification: Purified

Immunogen: Thymocytes and peripheral blood lymphocytes from a remission AML patient

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , ELISA - Reported in literature , Block - Reported in literature , Depletion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, and SPRD conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a 53-55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades