Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , ELISA - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and PE conjugate 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Buffer
100 tests in 1.0 mL of PBS/Sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The CD35 antigen is composed of four different allotypes having different molecular weights (A (or F) - 160 kDa, B (or S) - 190 kDa, C - 220 kDa, and D - 250 kDa). It is the receptor for C3b and is found on B cells, a subset of T cells, monocytes, neutrophils, follicular dendritic cells, erythrocytes and kidney glomelular podocytes. CD35 mediates neutrophil and monocyte phagocytosis of particles coated with C3b and/or C4b. This interaction also enhances phagocytosis mediated by the Fc receptors.