Luciferase antibody

Catalog No. ABIN1385113

This Rabbit Polyclonal antibody specifically detects Luciferase in ELISA, ICC, IF (p), IF (cc), IHC (fro) and IHC (p). It exhibits reactivity toward Firefly.

Quick Overview for Luciferase antibody (ABIN1385113)

Target: Luciferase

Reactivity: Firefly

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Luciferase Antibody

Cross-Reactivity: Tag

Cross-Reactivity (Details): Photinus pyralis (Common eastern firefly)

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Firefly Luciferase

Isotype: IgG

Application Details

Application Notes: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Luciferase

Target: Luciferase

Background:

Synonyms: Luciferin 4-monooxygenase

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Gene ID: 7100