CHN1 antibody (AA 151-250)

Catalog No. ABIN1385870

Product Details anti-CHN1 Antibody

Target: CHN1 (ARHGAP2) (rho GTPase Activating Protein 2 (ARHGAP2))

Binding Specificity: AA 151-250

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Alpha-chimerin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CHN1

Target: CHN1 (ARHGAP2) (rho GTPase Activating Protein 2 (ARHGAP2))

Alternative Name: CHN 1/Alpha-chimerin (ARHGAP2 Products)

Synonyms: ARHGAP2 antibody, CHN antibody, DURS2 antibody, NC antibody, RHOGAP2 antibody, 0610007I19Rik antibody, 0710001E19Rik antibody, 1700112L09Rik antibody, 2900046J01Rik antibody, AI413815 antibody, chimaerin antibody, wu:fr89a09 antibody, zgc:56160 antibody, zgc:92906 antibody, N-chimaerin antibody, chimerin 1 antibody, chimerin 1 L homeolog antibody, CHN1 antibody, Chn1 antibody, chn1.L antibody, chn1 antibody

Background:

Synonyms: A-chimaerin, Alpha-chimerin, ARHGAP2, CHIN_HUMAN, CHN, Chn1, N chimaerin, N chimerin, N-chimaerin, N-chimerin, NC, Rho GTPase-activating protein 2, RHOGAP2.

Background: The Rac-GAP chimaerin family member Alpha-chimaerin (also known as N-chimaerin or rho GTPase-activating protein 2) has two splice variants: Alpha1 and Alpha2. The ?-chimaerin variant is a neuron-specific, diacylglycerol-binding and GTPase-activating protein for ras-related protein Rac 1. This variant lacks the N-terminal SH2 domain that is present in the Alpha2 variant. By inactivating Rac 1, Alpha1-chimaerin plays a significant role in the regulation of dendritic growth during neuronal development. It is recruited to the plasma membrane by phospholipase C Beta-coupled cell surface receptors activating the downstream generation of DAG (diacylglycerol). Overexpression of Alpha1-chimaerin results in dendritic spine retraction and the loss of dendritic branches. In the presence of reduced neuronal activity, Alpha1-chimaerin expression is down-regulated resulting in an increase in spine growth and dendritic branching.