Glutathione Synthetase antibody (AA 81-160)

Catalog No. ABIN1386180

Product Details anti-Glutathione Synthetase Antibody

Target: Glutathione Synthetase (GSS)

Binding Specificity: AA 81-160

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutathione Synthetase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glutathione Syntase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Glutathione Synthetase

Target: Glutathione Synthetase (GSS)

Alternative Name: GSS/Glutathione Synthetase (GSS Products)

Synonyms: PRNP antibody, 6835 antibody, CG32497 antibody, CG33065 antibody, CG6835 antibody, Dmel\\CG6835 antibody, F21A20.90 antibody, F21A20_90 antibody, GLUTATHIONE SYNTHETASE antibody, GSHB antibody, glutathione synthetase 2 antibody, GSHS antibody, AI314904 antibody, GS-A/GS-B antibody, gssls-a antibody, glutathione synthetase antibody, Glutathione Synthetase antibody, glutathione synthetase 2 antibody, glutathione synthetase L homeolog antibody, GSS antibody, GSH2 antibody, Tola_1250 antibody, PC1_3711 antibody, Dd586_3633 antibody, Kvar_0701 antibody, Alvin_0197 antibody, Nhal_0215 antibody, BC1002_0254 antibody, Cseg_4017 antibody, Snov_3844 antibody, Aazo_1904 antibody, M301_2613 antibody, Bresu_0515 antibody, Isop_1460 antibody, Marme_4020 antibody, Thicy_0325 antibody, Sinme_3668 antibody, Mesop_0757 antibody, Gss antibody, gss.L antibody

Background:

Synonyms: Glutathione synthase; GSH S; GSH synthetase; GSH-S; GSHB_HUMAN; GSHS; GSS antibodyMGC14098; OTTHUMP00000030711.

Background: GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel -sheet, a parallel -sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel -sheet, a parallel -sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with ©-GCS and ending with GSS. In an ATP-dependent reaction, GSS produces Glutathione from ©-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamide increase the ex-pression of GSS, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GSS deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline. A missense mutation in the gene encoding GSS leads to a GSS deficiency restricted to erythrocytes, which causes only hemolytic anemia.-GCS and ending with GSS.

Pathways: Warburg Effect