p53 antibody (AA 1-393)

Catalog No. ABIN1386190

This Rabbit Polyclonal antibody specifically detects p53 in WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 4+ publications.

Quick Overview for p53 antibody (AA 1-393) (ABIN1386190)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-p53 Antibody

Binding Specificity: AA 1-393

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: Full length human p53 protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-p53 antibody (ABIN1386190)

Sola, Magrin, Pedrioli, Pinton, Salvadè, Papin, Paganetti: "Tau affects P53 function and cell fate during the DNA damage response." in: Communications biology, Vol. 3, Issue 1, pp. 245, (2021) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Zeng, Feng, Lin, Ma, Liu: "Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targetingSIRT1gene in diabetic cataract mice." in: Bioscience reports, Vol. 37, Issue 4, (2017) (PubMed).

Qin, Xiong, Liu, Yao, Zhou, Hua, Wang: "Effects of irreversible electroporation on cervical cancer cell lines in vitro." in: Molecular medicine reports, Vol. 14, Issue 3, pp. 2187-93, (2016) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background:

Synonyms: LFS1, p53, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, TP53, Transformation related protein 53, TRP53, Tumor protein p53, Tumour Protein p53.

Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect