DENND1B antibody (AA 241-350)

Catalog No. ABIN1387420

This Rabbit Polyclonal antibody specifically detects DENND1B in WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for DENND1B antibody (AA 241-350) (ABIN1387420)

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DENND1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DENND1B Antibody

Binding Specificity: AA 241-350

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DENND1B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Alternative Name: Dennd1b

Background:

Synonyms: C1ORF18, C1orf218, Chromosome 1 open reading frame 218, Connecdenn 2, DEN1B_HUMAN, DENN domain containing protein 1B, DENN domain-containing protein 1B, DENN MADD domain containing 1B, DENND1B, DKFZp547O0715, FAM31B, Family with sequence similarity 31 member B, FLJ20054, FLJ21179, FLJ33888, MGC103810, MGC27044, Protein FAM31B

Background: Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.Tissue specificity:Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.

Gene ID: 163486

UniProt: Q6P3S1