DENND1B antibody (AA 144-193)

Catalog No. ABIN6747143

Product Details anti-DENND1B Antibody

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Binding Specificity: AA 144-193

Reactivity: Human, Mouse, Rabbit, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DENND1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DENND1B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human DENND1B (Q6P3S1, NP_659414). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Stickleback, Zebrafish (100%), Bovine (92%), Rat, Panda, Lizard, Xenopus, Beetle (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Alternative Name: DENND1B (DENND1B Products)

Synonyms: C1ORF18 antibody, C1orf218 antibody, FAM31B antibody, 4632404N19Rik antibody, 4930467M19Rik antibody, 6820401H01Rik antibody, F730008N07Rik antibody, Fam31b antibody, si:ch211-195i21.1 antibody, DENN domain containing 1B antibody, DENN/MADD domain containing 1B antibody, DENND1B antibody, dennd1b antibody, Dennd1b antibody

Background: Name/Gene ID: DENND1B

Synonyms: DENND1B, C1ORF18, C1orf218, Connecdenn 2, DENN/MADD domain containing 1B, Protein FAM31B, FAM31B, RP11-53I24.2

Gene ID: 163486

NCBI Accession: NP_659414

UniProt: Q6P3S1