HIP1 antibody (AA 401-500)

Catalog No. ABIN1387494

Product Details anti-HIP1 Antibody

Target: HIP1 (Huntingtin Interacting Protein 1 (HIP1))

Binding Specificity: AA 401-500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HIP1

Target: HIP1 (Huntingtin Interacting Protein 1 (HIP1))

Alternative Name: HIP1 (HIP1 Products)

Synonyms: HIP-I antibody, ILWEQ antibody, CG10971 antibody, Dmel\\CG10971 antibody, Hip1R antibody, 2610109B09Rik antibody, A930014B11Rik antibody, E130315I21Rik antibody, mKIAA4113 antibody, huntingtin interacting protein 1 antibody, Huntingtin interacting protein 1 antibody, HIP1 antibody, Hip1 antibody, hip1 antibody

Background:

Synonyms: Huntingtin Interacting Protein HIP1, HIP I, HIP-1, HIP-I, hip1, HIP1/PDGFRB fusion gene, HIP1/PDGFRB fusion gene, included, HIP1_HUMAN, HIPI, Huntingtin interacting protein 1, Huntingtin-interacting protein 1, Huntingtin-interacting protein I, ILWEQ, KIAA4113, MGC126506, MGC27616, mKIAA4113.

Background: Huntington disease is associated with the expansion of a polyglutamine tract, greater than 35 repeats, in the HD gene product huntingtin. HIP1 (huntingtin-interacting protein 1), a membrane-associated protein, binds specifically to the N-terminus of human huntingtin. HIP1 is ubiquitously expressed in different brain regions at low levels, and exhibits nearly identical subcellular fractionation as huntingtin. The huntingtin-HIP1 interaction is restricted to the brain and is inversely correlated to the polyglutamine length in the huntingtin, suggesting that loss of normal huntingtin-HIP1 interaction may compromise the membrane-cytoskeletal integrity in the brain. HIP1 contains an endocytic multidomain protein with a C-terminal Actin-binding domain, a central coiled-coil forming region and an N-terminal ENTH domain. HIP1 may be involved in vesicle trafficking, the structural integrity of HIP1 is crucial for maintenance of normal vesicle size in vivo. HIP12 is a non-proapoptotic member of the HIP gene family that is expressed in the brain and shares a similar subcellular distribution pattern with HIP1. However, HIP12 differs from HIP1 in its pattern of expression at both the mRNA and protein level. HIP12 does not directly interact with huntingtin but can interact with HIP1.

Pathways: Positive Regulation of Endopeptidase Activity