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E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 410-460) Primary Antibody

CDH1 Reactivity: Human, Mouse, Rat FACS, IF (cc), IF (p), IHC (p), WB Host: Rabbit Polyclonal
Pubmed (14) Independent Validation (1)
Catalog No. ABIN1387847
$339.90
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100 μL
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  • Target
    Binding Specificity
    AA 410-460
    Reactivity
    Human, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This E-cadherin antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human E-cadherin
    Isotype
    IgG
  • Application Notes
    WB 1:100-1000
    FCM 1:20-100
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029768 (Flow Cytometry)
    'Independent Validation' Badge
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029768
    Date
    07/22/2014
    Antigen
    Lot Number
    130902
    Method validated
    Flow Cytometry
    Positive Control
    MCF-7 cells
    Negative Control
    SH-SY5Y cells
    Notes
    A weak but specific signal is observed in the positive control MCF7 cells stained with anti-E-Cadherin plus secondary antibody compared with isotype, secondary only and unstained cells. No staining was observed in the negative control SH-SY5Y cells as expected.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
    • Catalog number: ABIN1387847
    • Lot number: 130902
    • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
    Secondary Antibody
    • Antibody: Goat anti-rabbit IgG-Alexa 647
    • Dilution: 1:500 in 1X PBS containing 0.5% BSA
    Full Protocol
    • Positive and negative control cells were cultured in DMEM + 10% FBS. - Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
    • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
    • Cells were incubated for 30 min on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
    • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Propidium Iodide (PI) was added to discern live cells from dead cells.
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Experimental Notes
    - The data displayed is gated on PI negative cells.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C
    Expiry Date
    12 months
  • Guo, Gao, Sui, Jiao, Sun, Fu, Jin: "miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells." in: Clinical and experimental pharmacology & physiology, Vol. 46, Issue 2, pp. 144-152, 2019 (PubMed). Method employed by authors: Western Blotting (WB)

    Yun, Gao, Yue, Guo, Li, Sang: "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." in: Environmental science & technology, Vol. 51, Issue 19, pp. 11401-11411, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Fang, Liu, Wu, Liu, Pan, Li: "Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma." in: OncoTargets and therapy, Vol. 11, pp. 5647-5655, 2018 (PubMed).

    Lin, Zhang, Dai, Zhang, Zhang, Xue, Wu: "TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway." in: Journal of Cancer, Vol. 9, Issue 23, pp. 4430-4439, 2018 (PubMed). Method employed by authors: Immunofluorescence (Cultured Cells) (IF (cc))

    Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

    Borin, Shankar, Angara, Rashid, Jain, Iskander, Ara, Lebedyeva, Korkaya, Achyut, Arbab: "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178830, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: tumor

    Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

    Wang, Nikhil, Viccaro, Chang, Jacobsen, Sandusky, Shah: "The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma." in: Journal of cell science, Vol. 130, Issue 6, pp. 1078-1093, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

    Short, Kasper, van der Aa, Andeweg, Zaaraoui-Boutahar, Goeijenbier, Richard, Herold, Becker, Scott, Limpens, Koster, Bárcena, Fouchier, Kirkpatrick, Kuiken: "Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions." in: The European respiratory journal, Vol. 47, Issue 3, pp. 954-66, 2016 (PubMed). Method employed by authors: Immunofluorescence (Cultured Cells) (IF (cc))

    Hu, Duan, Li, Wang, Wang, Chu, Sun, Liu, Wang, Lu, Wen: "Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153409, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

    Neelam, Brooks, Cammarata: "Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3?, epithelial to mesenchymal transition, and mitochondrial depolarization." in: Molecular vision, Vol. 20, pp. 1758-75, 2015 (PubMed). (Sample species: Human).

    Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, 2015 (PubMed).

    Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, 2014 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

    Cai: "Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma." in: Experimental and therapeutic medicine, Vol. 6, Issue 6, pp. 1489-1493, 2013 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

  • Target
    Alternative Name
    E Cadherin (CDH1 Antibody Abstract)
    Synonyms
    Arc-1, CD324, CDHE, ECAD, LCAM, UVO, E-cadherin, cdh1, si:dz180o5.2, uvo, lcam, E-Cad, XBcad, l-cam, xcdh1, cdhc-A, xb-cad, XTCAD-1, c-cadherin, cadherin-1, uvomorulin, XB-cadherin, arc-1, cd324, cdhe, ecad, AA960649, E-cad, Ecad, L-CAM, Um, cadherin 1, si:busm1-180o5.2, cadherin 1, type 1, E-cadherin (epithelial), cadherin 1, type 1 S homeolog, CDH1, si:busm1-180o5.2, cdh1, Cdh1, cdh1.S
    Background

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

    Subcellular location: Extracellular

    Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

    Gene ID
    999
    UniProt
    P12830
    Pathways
    Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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