E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 410-460)

Details for Product anti-CDH1 Antibody No. ABIN1387847
Antigen
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • cdh1
  • si:dz180o5.2
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • arc-1
  • cd324
  • cdhe
  • ecad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • cadherin 1
  • si:busm1-180o5.2
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin 1, type 1 S homeolog
  • CDH1
  • si:busm1-180o5.2
  • cdh1
  • Cdh1
  • cdh1.S
Epitope
AA 410-460
27
22
17
15
15
15
13
10
9
8
8
6
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
581
217
192
35
16
16
9
9
9
7
6
6
4
3
3
3
3
3
3
2
1
Host
Rabbit
332
282
14
5
2
Clonality
Polyclonal
Conjugate
This E-cadherin antibody is un-conjugated
38
33
16
15
13
10
10
10
10
10
9
8
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
465
245
195
153
143
117
69
44
37
32
16
14
13
12
10
10
7
6
4
3
1
Options
'Independent Validation' Badge
Lot Number 130902
Method validated Flow Cytometry
Positive Control MCF-7 cells
Negative Control SH-SY5Y cells
Notes A weak but specific signal is observed in the positive control MCF7 cells stained with anti-E-Cadherin plus secondary antibody compared with isotype, secondary only and unstained cells. No staining was observed in the negative control SH-SY5Y cells as expected.
Primary Antibody
  • Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
  • Catalog number: ABIN1387847
  • Lot number: 130902
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotype Control Antibody
  • Antibody: Rabbit IgG
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Secondary Antibody
  • Antibody: Goat anti-rabbit IgG-Alexa 647
  • Dilution: 1:500 in 1X PBS containing 0.5% BSA
Controls
  • Positive control: MCF-7 cells
  • Negative control: SH-SY5Y cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-Alexa 647 to confirm no background signal produced from secondary antibody alone
Protocol
  • Positive and negative control cells were cultured in DMEM + 10% FBS. - Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Propidium Iodide (PI) was added to discern live cells from dead cells.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Experimental Notes - The data displayed is gated on PI negative cells.
Validation Images
Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Figure 1: Positive control MCF7 cells. The red histogram is unstained cells, the blue...
Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Figure 2: Negative control SH-SY5Y cells. The red histogram is unstained cells, the b...
Immunogen KLH conjugated synthetic peptide derived from human E-cadherin
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others E-cadherin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name E Cadherin (CDH1 Antibody Abstract)
Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subcellular location: Extracellular

Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

Gene ID 999
UniProt P12830
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E ...
Image no. 2 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.5 Co...
Image no. 3 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by Independent Validation badge 29768.Histogram of MCF7 cells stained ...
Image no. 4 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD3...
Image no. 5 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 i...
Image no. 6 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD3...
Image no. 7 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by One World Lab validation program. U138 cells probed with Rabbit Ant...
Image no. 8 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
Image no. 9 for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at...
Product cited in: Guo, Gao, Sui, Jiao, Sun, Fu, Jin: "miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells." in: Clinical and experimental pharmacology & physiology, Vol. 46, Issue 2, pp. 144-152, 2019 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Yun, Gao, Yue, Guo, Li, Sang: "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." in: Environmental science & technology, Vol. 51, Issue 19, pp. 11401-11411, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Fang, Liu, Wu, Liu, Pan, Li: "Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma." in: OncoTargets and therapy, Vol. 11, pp. 5647-5655, 2018 (PubMed).

Lin, Zhang, Dai, Zhang, Zhang, Xue, Wu: "TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway." in: Journal of Cancer, Vol. 9, Issue 23, pp. 4430-4439, 2018 (PubMed). Method employed by authors: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Cultured Cells) (IF (cc))

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Borin, Shankar, Angara, Rashid, Jain, Iskander, Ara, Lebedyeva, Korkaya, Achyut, Arbab: "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178830, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: tumor

Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Wang, Nikhil, Viccaro, Chang, Jacobsen, Sandusky, Shah: "The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma." in: Journal of cell science, Vol. 130, Issue 6, pp. 1078-1093, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Short, Kasper, van der Aa, Andeweg, Zaaraoui-Boutahar, Goeijenbier, Richard, Herold, Becker, Scott, Limpens, Koster, Bárcena, Fouchier, Kirkpatrick, Kuiken: "Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions." in: The European respiratory journal, Vol. 47, Issue 3, pp. 954-66, 2016 (PubMed). Method employed by authors: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Cultured Cells) (IF (cc))

Hu, Duan, Li, Wang, Wang, Chu, Sun, Liu, Wang, Lu, Wen: "Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153409, 2016 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB)

Neelam, Brooks, Cammarata: "Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3?, epithelial to mesenchymal transition, and mitochondrial depolarization." in: Molecular vision, Vol. 20, pp. 1758-75, 2015 (PubMed). (Sample species: Human).

Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, 2015 (PubMed).

Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, 2014 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Cai: "Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma." in: Experimental and therapeutic medicine, Vol. 6, Issue 6, pp. 1489-1493, 2013 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

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