E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 410-460)

Details for Product anti-CDH1 Antibody No. ABIN1387847, Supplier: Log in to see
Antigen
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • arc-1
  • cd324
  • cdhe
  • ecad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • cadherin 1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin 1, type 1 S homeolog
  • CDH1
  • cdh1
  • Cdh1
  • cdh1.S
Alternatives
anti-Human E-cadherin antibody for Flow Cytometry
Epitope
AA 410-460
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28
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2
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2
1
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
808
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9
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1
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Host
Rabbit
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2
Clonality
Polyclonal
Conjugate
This E-cadherin antibody is un-conjugated
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Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Lot Number 130902
Method validated Flow Cytometry
Positive Control MCF-7 cells
Negative Control SH-SY5Y cells
Notes A weak but specific signal is observed in the positive control MCF7 cells stained with anti-E-Cadherin plus secondary antibody compared with isotype, secondary only and unstained cells. No staining was observed in the negative control SH-SY5Y cells as expected.
Primary Antibody
  • Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
  • Catalog number: ABIN1387847
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  • Supplier catalog number: Log in to see
  • Lot number: 130902
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotype Control Antibody
Secondary Antibody
  • Antibody: Goat anti-rabbit IgG-Alexa 647
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  • Dilution: 1:500 in 1X PBS containing 0.5% BSA
Controls
  • Positive control: MCF-7 cells
  • Negative control: SH-SY5Y cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-Alexa 647 to confirm no background signal produced from secondary antibody alone
Protocol
  • Positive and negative control cells were cultured in DMEM + 10% FBS. - Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Propidium Iodide (PI) was added to discern live cells from dead cells.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Experimental Notes - The data displayed is gated on PI negative cells.
Validation Images
Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Figure 1: Positive control MCF7 cells. The red histogram is unstained cells, the blue...
Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Figure 2: Negative control SH-SY5Y cells. The red histogram is unstained cells, the b...
Immunogen KLH conjugated synthetic peptide derived from human E-cadherin
Isotype IgG
Purification Purified by Protein A.
Alternative Name E Cadherin (CDH1 Antibody Abstract)
Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subcellular location: Extracellular

Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

Gene ID 999
UniProt P12830
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E ...
Immunofluorescence (IF) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD3...
Immunofluorescence (IF) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD3...
Western Blotting (WB) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at...
Flow Cytometry (FACS) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.5 Co...
Flow Cytometry (FACS) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by Independent Validation badge 29768.Histogram of MCF7 cells stained ...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 i...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by One World Lab validation program. U138 cells probed with Rabbit Ant...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
Product cited in: Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Neelam, Brooks, Cammarata: "Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3?, epithelial to mesenchymal transition, and mitochondrial depolarization." in: Molecular vision, Vol. 20, pp. 1758-75, 2015 (PubMed).

Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, 2015 (PubMed).

Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, 2014 (PubMed).

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