HUNK antibody (AA 71-115)

Catalog No. ABIN1387914

The Rabbit Polyclonal anti-HUNK antibody (ABIN1387914) specifically detects HUNK in WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for HUNK antibody (AA 71-115) (ABIN1387914)

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HUNK Antibody

Binding Specificity: AA 71-115

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Serine/threonine-protein kinase MAK-V

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HUNK

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Alternative Name: Serine/threonine-protein kinase MAK-V

Background:

Synonyms: B19, Hormonally up-regulated neu tumor-associated kinase, Hormonally upregulated neu associated, Hormonally upregulated neu associated kinase, Hormonally upregulated neu tumor associated kinase, HUNK, HUNK_HUMAN, MAKV, Serine/threonine-protein kinase MAK-V.

Background: The HUNK (hormonally upregulated Neu-associated kinase) protein, also designated MAK-V in mouse, has been identified as a novel SNF1-related serine/threonine kinase. The human HUNK gene localizes to chromosome 21q22 and encodes a protein with nucleocytoplasmic distribution and localizes to the centrosome. Overexpression of the HUNK protein associates with approximately 50 % of breast carcinomas, and may provide diagnostic-prognostic value as a molecular marker. Serine/threonine-protein kinase SNF1-like kinase 2 (SIK) phosphorylates Ser-794 of IRS1 in insulin-stimulated adipocytes, which may modulate the efficiency of insulin signal transduction. SIK is activated by phosphorylation on Thr-175 by STK11 in complex with STE20-related adapter- and CAB39.