IDH1 antibody (AA 70-115) (FITC)

Catalog No. ABIN1403780

The Rabbit Polyclonal anti-IDH1 antibody has been validated for WB and IF (p). It is suitable to detect IDH1 in samples from Human, Mouse and Rat.

Quick Overview for IDH1 antibody (AA 70-115) (FITC) (ABIN1403780)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-IDH1 Antibody (FITC)

Binding Specificity: AA 70-115

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IDH1/Isocitrate dehydrogenase

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background:

Synonyms: Cytosolic NADP isocitrate dehydrogenase, Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDCD, IDH, IDHC, Idh1, IDHC_HUMAN, IDP, IDPC, Isocitrate dehydrogenase [NADP] cytoplasmic, Isocitrate dehydrogenase 1 NADP+ soluble, NADP dependent isocitrate dehydrogenase cytosolic, NADP dependent isocitrate dehydrogenase peroxisomal, NADP+-specic ICDH, Oxalosuccinate decarboxylase, PICD.

Background: The Isocitrate dehydrogenase cytoplasmic enzyme is a homodimer of 416 residues that belongs to the isocitrate and isopropylmalate dehydrogenases family. IDHC catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming ?ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of ?ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Ca2+ can bind to IDHC as a complex with isocitrate, acting as a competitive inhibitor of Mg2+. The IDHC enzyme is inactivated by phosphorylation at Ser-113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock. IDHC is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.

Gene ID: 3417

UniProt: O75874

Pathways: Warburg Effect