RFC1 antibody (AA 401-500) (Cy5.5)

Catalog No. ABIN1408367

The Rabbit Polyclonal anti-RFC1 antibody is suitable to detect RFC1 in samples from Human and Mouse. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for RFC1 antibody (AA 401-500) (Cy5.5) (ABIN1408367)

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC1 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RFC1 Antibody (Cy5.5)

Binding Specificity: AA 401-500

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RFC1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RFC1

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Alternative Name: RFC1

Background:

Synonyms: DNA binding Protein PO GA, DNA-binding protein PO-GA, MHC binding factor beta, MHCBFB, RECC1, Replication factor C 140 kDa subunit, Replication factor C, Replication factor C large subunit, Replication factor C subunit 1, Replication factor C1, RF-C 140 kDa subunit, RFC1, RFC1_HUMAN, RFC140, RFC140 Replication Factor C 140 kDa subunit, A1 140 kDa subunit, A1 P145 Activator 1 large subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1.

Background: Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (including RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC1 is a substrate for caspase-3 in vitro and is cleaved by a caspase-3-like protease during FAS-mediated apoptosis. In addition, phosphorylation of the PCNA binding domain of RFC1 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibits DNA synthesis. The human RFC1 gene maps to chromosome 4p14 and encodes the RFC1 subunit.

Gene ID: 5981

UniProt: P35251

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport