OTX2 antibody (AA 15-105) (Cy7)

Catalog No. ABIN1410108

This anti-OTX2 antibody is a Rabbit Polyclonal antibody detecting OTX2 in WB, IF (cc) and IF (p). Suitable for Rat.

Quick Overview for OTX2 antibody (AA 15-105) (Cy7) (ABIN1410108)

Target: OTX2 (Orthodenticle Homeobox 2 (OTX2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTX2 antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-OTX2 Antibody (Cy7)

Binding Specificity: AA 15-105

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human OTX2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for OTX2

Target: OTX2 (Orthodenticle Homeobox 2 (OTX2))

Alternative Name: OTX2

Background:

Synonyms: CPHD6, Homeobox protein OTX2, MCOPS 5, MCOPS5, MGC45000, Orthodenticle 2, Orthodenticle homeobox 2, Orthodenticle homolog 2 Drosophila, Orthodenticle homolog 2, Orthodenticle2, Otx 2, otx2, OTX2_HUMAN.

Background: Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.

Pathways: Dopaminergic Neurogenesis