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RNF135 antibody (AA 288-360) (HRP)

The Rabbit Polyclonal anti-RNF135 antibody has been validated for WB, ELISA, IHC (fro) and IHC (p). It is suitable to detect RNF135 in samples from Human.
Catalog No. ABIN1411016

Quick Overview for RNF135 antibody (AA 288-360) (HRP) (ABIN1411016)

Target

See all RNF135 Antibodies
RNF135 (Ring Finger Protein 135 (RNF135))

Reactivity

  • 35
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 34
  • 1
Rabbit

Clonality

  • 35
Polyclonal

Conjugate

  • 17
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This RNF135 antibody is conjugated to HRP

Application

  • 35
  • 14
  • 13
  • 13
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 14
    • 8
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 288-360

    Predicted Reactivity

    Human,Mouse,Rat,Dog,Cow

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human RNF135

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    RNF135 (Ring Finger Protein 135 (RNF135))

    Alternative Name

    RNF135

    Background

    Synonyms: L13, MGC13061, ring finger protein 135, RN135_HUMAN .

    Background: The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF135 (RING finger protein 135), also known as L13, is a 432 amino acid protein that contains one RING-type zinc finger and one SPRY domain. Via its RING-type zinc finger, RNF135 may play a role in transcriptional regulation and protein degradation events. Defects in the gene encoding RNF135 are the cause of RNF135-related overgrowth syndrome which is characterized by learning disabilities, facial dysmorphism and increased weight and height. Multiple isoforms of RNF135 exist due to alternative splicing events.

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