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CHEK2 antibody (AA 101-200) (HRP)

The Rabbit Polyclonal anti-CHEK2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect CHEK2 in samples from Human.
Catalog No. ABIN1411244

Quick Overview for CHEK2 antibody (AA 101-200) (HRP) (ABIN1411244)

Target

See all CHEK2 Antibodies
CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity

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  • 1
Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This CHEK2 antibody is conjugated to HRP

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

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    AA 101-200

    Predicted Reactivity

    Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human Rad53/SPK1

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    CHEK2 (Checkpoint Kinase 2 (CHEK2))

    Alternative Name

    Rad53/SPK1

    Background

    Synonyms: CHEK2 homolog, CHK2 homolog, MEC2, RAD53, RAD53_YEAST, Serine protein kinase 1, Serine-protein kinase 1, Serine/threonine-protein kinase RAD53, SPK1.

    Background: DNA damage results in the arrest of cell cycle progression, allowing the damaged DNA to be repaired prior to replication. Checkpoints exist at several cell cycle phase transitions to maintain this genetic integrity. Rad9, Rad17, Rad24 and Mec3 are involved in activating the G1 and G2 checkpoints (14). Pol2 (also known as Dun2), encoding the catalytic subunit of DNA polymerase epsilon, plays a role in activating the S phase checkpoint (5). The protein kinase Rad53 (also designated Spk1, Mec2 or Sad1) is essential for both G2 and S phase arrest. Activation of Rad53 is regulated by Mec1 (also known as Esr1 and Sad3), a homolog of the human ATM protein (6). Pds1 and Mad2 both regulate checkpoints associated with incomplete spindle replication (7,8). Dun1, another protein kinase, plays a role in transducing the DNA damage signal (9).

    Pathways

    p53 Signaling, Apoptosis, Cell Division Cycle
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