HUNK antibody (AA 71-115) (HRP)

Catalog No. ABIN1412408

The Rabbit Polyclonal anti-HUNK antibody is suitable to detect HUNK in samples from Human. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for HUNK antibody (AA 71-115) (HRP) (ABIN1412408)

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUNK antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HUNK Antibody (HRP)

Binding Specificity: AA 71-115

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Serine/threonine-protein kinase MAK-V

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HUNK

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Alternative Name: Serine/threonine-protein kinase MAK-V

Background:

Synonyms: B19, Hormonally up-regulated neu tumor-associated kinase, Hormonally upregulated neu associated, Hormonally upregulated neu associated kinase, Hormonally upregulated neu tumor associated kinase, HUNK, HUNK_HUMAN, MAKV, Serine/threonine-protein kinase MAK-V.

Background: The HUNK (hormonally upregulated Neu-associated kinase) protein, also designated MAK-V in mouse, has been identified as a novel SNF1-related serine/threonine kinase. The human HUNK gene localizes to chromosome 21q22 and encodes a protein with nucleocytoplasmic distribution and localizes to the centrosome. Overexpression of the HUNK protein associates with approximately 50 % of breast carcinomas, and may provide diagnostic-prognostic value as a molecular marker. Serine/threonine-protein kinase SNF1-like kinase 2 (SIK) phosphorylates Ser-794 of IRS1 in insulin-stimulated adipocytes, which may modulate the efficiency of insulin signal transduction. SIK is activated by phosphorylation on Thr-175 by STK11 in complex with STE20-related adapter- and CAB39.