UBE2M antibody (AA 1-100) (HRP)

Catalog No. ABIN1416634

This Rabbit Polyclonal antibody specifically detects UBE2M in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Xenopus laevis.

Quick Overview for UBE2M antibody (AA 1-100) (HRP) (ABIN1416634)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Reactivity: Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UBE2M Antibody (HRP)

Binding Specificity: AA 1-100

Cross-Reactivity: Xenopus laevis

Predicted Reactivity: Human,Mouse,Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2M

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M

Background:

Synonyms: hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, UBC-RS2, UBC12, UBC12_HUMAN, ube2m, ubiquitin carrier protein M, Ubiquitin conjugating enzyme E2 M, Ubiquitin-conjugating enzyme E2 M, ubiquitin-protein ligase M, yeast UBC12 homolog.

Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene ID: 9040