UBE2M antibody (Internal Region)

Catalog No. ABIN6265852

The Rabbit Polyclonal anti-UBE2M antibody (ABIN6265852) specifically detects UBE2M in WB, IHC, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBE2M antibody (Internal Region) (ABIN6265852)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-UBE2M Antibody

Binding Specificity: Internal Region

Purpose: UBE2M Antibody

Specificity: UBE2M Antibody detects endogenous levels of total UBE2M.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human UBE2M, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M

Background:

Description: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene: UBE2M

Molecular Weight: 21 kDa

Gene ID: 9040

UniProt: P61081