UBE2M antibody (AA 169-180)

Catalog No. ABIN6656076

Product Details anti-UBE2M Antibody

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Binding Specificity: AA 169-180

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Chimpanzee, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)

Purification: UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human UBC12 protein. However, 100% homology is on record for this protein from human, chimpanzee, frog, mouse, rat and dog as indicated by BLAST analysis.  Reactivity with UBC12 proteins from other sources is not known.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 169-180 of Human UBC12 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: UBC12 Antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-UBE2M antibody (ABIN6656076)

Liu, Reitsma, Mamrosh, Zhang, Straube, Deshaies: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." in: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).

Kolokoltsova, Domina, Kolokoltsov, Davey, Weaver, Watowich: "Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling." in: Virology, Vol. 377, Issue 1, pp. 133-42, (2008) (PubMed).

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBC12 (ube2m Products)

Synonyms: DDBDRAFT_0204633 antibody, DDBDRAFT_0238042 antibody, DDB_0204633 antibody, DDB_0238042 antibody, UBC-RS2 antibody, UBC12 antibody, hUbc12 antibody, 2510040H03Rik antibody, Ubc-rs2 antibody, ubiquitin conjugating enzyme E2M antibody, ubiquitin-conjugating enzyme E2M antibody, ubiquitin conjugating enzyme E2 M antibody, NEDD8-conjugating enzyme antibody, ubiquitin conjugating enzyme E2M S homeolog antibody, ube2m antibody, LOC692934 antibody, UBE2M antibody, Ube2m antibody, ube2m.S antibody

Background:

Synonyms: rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody

Background: UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family.  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.

Gene Name: UBE2M

Gene ID: 9040, 4507791

UniProt: P61081