SIRT5 antibody (AA 120-170) (HRP)

Catalog No. ABIN1420690

This Rabbit Polyclonal antibody specifically detects SIRT5 in WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SIRT5 antibody (AA 120-170) (HRP) (ABIN1420690)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT5 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SIRT5 Antibody (HRP)

Binding Specificity: AA 120-170

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIRT5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Alternative Name: SIRT5

Background:

Synonyms: SIR2L5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIRT5

Background: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity, however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.

Gene ID: 23408

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interactome