EGF antibody (PE-Cy7)

Catalog No. ABIN1430675

The Rabbit Polyclonal anti-EGF antibody is suitable to detect EGF in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for EGF antibody (PE-Cy7) (ABIN1430675)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is conjugated to PE-Cy7

Application: Western Blotting (WB)

Product Details anti-EGF Antibody (PE-Cy7)

Cross-Reactivity: Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: Octameric MAP derived from mouse EGF

Isotype: IgG

Application Details

Application Notes: FCM(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background:

Synonyms: AI790464, Pro-epidermal growth factor, EGF

Background: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).

Molecular Weight: 5.8/133kDa

Gene ID: 13645

UniProt: P01132

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation