GST antibody

Catalog No. ABIN1440042

Cited in 1 publication. The Goat Polyclonal anti-GST antibody (ABIN1440042) specifically detects GST in WB. The antibody is reactive with Tag samples.

Quick Overview for GST antibody (ABIN1440042)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Tag

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GST Antibody

Purpose: anti-GST

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMYDALDV VLYMDPMCLD AFPKLVCFKK RIEAIPQIDK YLKSSKYIAW PLQGWQATFG GGDHPPKSDL VPRGSPEFPG RLERPHR

Specificity: In lysates of E. coli cells transformed with pGEX plasmid detects a band of 26 kDa by Western blot.

Characteristics: Gluthatione-S-Transferase

Purification: Epitope affinity purified

Immunogen: Purified recombinant protein produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GST antibody (ABIN1440042)

Domingues, Ismail, Charro, Rodríguez-Escudero, Holden, Molina, Cid, Mota: "The Salmonella effector SteA binds phosphatidylinositol 4-phosphate for subcellular targeting within host cells." in: Cellular microbiology, Vol. 18, Issue 7, pp. 949-69, (2017) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST)

Target Type: Tag

Background: Goat polyclonal antibody to GST (Glutathione-S-Transferase). GST is a 26 kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in GST plasmid expression vectors as a fusion protein with foreign protein.

Molecular Weight: 26 kDa