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Vimentin antibody (pSer50)

This Rat Monoclonal antibody specifically detects Vimentin in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN1449160

Quick Overview for Vimentin antibody (pSer50) (ABIN1449160)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 348
  • 128
  • 126
  • 63
  • 61
  • 49
  • 43
  • 38
  • 31
  • 22
  • 21
  • 13
  • 13
  • 8
  • 8
  • 6
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat

Host

  • 183
  • 154
  • 10
  • 6
  • 5
  • 5
  • 2
  • 2
Rat

Clonality

  • 184
  • 182
Monoclonal

Conjugate

  • 215
  • 20
  • 13
  • 12
  • 9
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
This Vimentin antibody is un-conjugated

Application

  • 306
  • 152
  • 117
  • 100
  • 91
  • 61
  • 57
  • 40
  • 39
  • 35
  • 28
  • 27
  • 22
  • 8
  • 6
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF)

Clone

TM50
  • Binding Specificity

    • 32
    • 31
    • 17
    • 16
    • 11
    • 10
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer50

    Sequence

    CPSTSRpSLY SSS

    Specificity

    This antibody reacts with vimentin phosphorylated at Ser50.

    Cross-Reactivity (Details)

    Species reactivity (tested):Human, mouse, rat

    Purification

    Protein G agarose

    Immunogen

    KLH-PV50 peptide

    Isotype

    IgG2a
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM

    Gene ID

    7431

    NCBI Accession

    NP_003371

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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