SQSTM1 antibody (C-Term)

Catalog No. ABIN1449619

This Rabbit Polyclonal antibody specifically detects SQSTM1 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for SQSTM1 antibody (C-Term) (ABIN1449619)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 324-353, C-Term

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human SQSTM1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background: SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.Synonyms: EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62

Molecular Weight: 47687 Da

Gene ID: 8878

NCBI Accession: NP_001135770

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy