KEAP1 antibody (C-Term)

Catalog No. ABIN1449975

The Rabbit Polyclonal anti-KEAP1 antibody (ABIN1449975) specifically detects KEAP1 in WB, IF and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for KEAP1 antibody (C-Term) (ABIN1449975)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-KEAP1 Antibody

Binding Specificity: C-Term

Purification: Affinity chromatography purified via peptide column

Immunogen: A 19 amino acid synthetic peptide near the carboxy terminus of Human KEAP1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-kB activity by targeting IKKbeta degradation. Mutation of the KEAP1 gene is found in lung cancer.Synonyms: Cytosolic inhibitor of Nrf2, INRF2, INrf2, KEAP1, KIAA0132, KLHL19, Kelch-like ECH-associated protein 1, Kelch-like protein 19

Molecular Weight: 69 kDa

Gene ID: 9817

NCBI Accession: NP_987096

Pathways: Maintenance of Protein Location