Shugoshin antibody (pSer14)

Catalog No. ABIN1450506

The Rabbit Polyclonal anti-Shugoshin antibody (ABIN1450506) specifically detects Shugoshin in ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Shugoshin antibody (pSer14) (ABIN1450506)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Shugoshin Antibody

Binding Specificity: AA 1-50, pSer14

Purpose: Sgo1 (phospho Ser14) Antibody

Specificity: Phospho-Sgo1 (S14) Polyclonal Antibody detects endogenous levels of Sgo1 protein only when phosphorylated at S14.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human SGO1 around the phosphorylation site of Ser14. AA range:1-50

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: SGO1

Background:

Synonyms: SGOL1, SGO1, Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85

Background: The protein encoded byThis gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibro

Gene Name: SGOL1

Protein Name: Shugoshin-like 1

Molecular Weight: 64 kDa

Gene ID: 151648

OMIM: 609168

UniProt: Q5FBB7

Pathways: Maintenance of Protein Location