14-3-3 sigma/SFN antibody (AA 41-90)

Catalog No. ABIN1450896

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody (ABIN1450896) specifically detects 14-3-3 sigma/SFN in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 41-90) (ABIN1450896)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 41-90

Purpose: 14-3-3 σ Antibody

Specificity: 14-3-3 σ Polyclonal Antibody detects endogenous levels of 14-3-3 σ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human SFN. AA range:41-90

Isotype: IgG

Application Details

Application Notes: IHC: 100-300.WB 1:500 - 1:2000. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 sigma

Background:

Synonyms: SFN, HME1, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

Background: function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.,function:p53-regulated inhibitor of G2/M progression.,similarity:Belongs to the 14-3-3 family.,subcellular location:May be secreted by a non-classical secretory pathway.,subunit:Homodimer. Interacts with KRT17 (By similarity). Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN.,tissue specificity:Present mainly in tissues enriched in stratified squamous keratinising epithelium.,

Gene Name: SFN

Protein Name: 14-3-3 protein sigma

Molecular Weight: 27 kDa

Gene ID: 2810

OMIM: 601290

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction