RHOB antibody (AA 99-148)

Catalog No. ABIN1451565

The Rabbit Polyclonal anti-RHOB antibody (ABIN1451565) specifically detects RHOB in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RHOB antibody (AA 99-148) (ABIN1451565)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RHOB Antibody

Binding Specificity: AA 99-148

Purpose: Rho B Antibody

Specificity: Rho B Polyclonal Antibody detects endogenous levels of Rho B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human RHOB. AA range:99-148

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Alternative Name: RHOB

Background:

Synonyms: RHOB, ARH6, ARHB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6

Background: function:Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.,miscellaneous:RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.,PTM:Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.,similarity:Belongs to the small GTPase superfamily. Rho family.,subcellular location:Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.,subunit:Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13.,

Gene Name: RHOB

Protein Name: Rho-related GTP-binding protein RhoB

Molecular Weight: 22 kDa

Gene ID: 388

OMIM: 165370

UniProt: P62745