RHOB antibody (AA 70-150)

Catalog No. ABIN7222072

The Rabbit Polyclonal anti-RHOB antibody (ABIN7222072) specifically detects RHOB in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RHOB antibody (AA 70-150) (ABIN7222072)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RHOB Antibody

Binding Specificity: AA 70-150

Purpose: Rabbit Anti-Rho B Polyclonal Antibody

Specificity: Rho B Polyclonal Antibody detects endogenous levels of Rho B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Rho B at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Alternative Name: Rho B

Background: RHOB, ARH6, ARHB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules.

Molecular Weight: 22kD

Gene ID: 388

UniProt: P62745